The Division I regional football alignments for the 2015 and 2016 season were approved by the TSSAA Board of Control on November 13, 2014.
Class 3A – Region 5
- Christ Presbyterian Academy
- East Nashville Magnet School
- Goodpasture Christian School
- Greenbrier High School
- Stratford High School
- White Hour Heritage High School
- Whites Creek High School
Class 3A – Region 6
- Camden Central High School
- Cheatham Co. Central High School
- East Hickman High School
- Fairview High School
- Harpeth High School
- Stewart Co. High School
- Sycamore High School
Class 4A – Region 4
- Giles Co. High School
- Lawrence Co. High School
- Marshall Co. High School
- Page High School
- Spring Hill High School
- Tullahoma High School
Class 5A – Region 5
- Columbia Central High School
- Franklin Co. High School
- Independence High School
- LaVergne High School
- Lincoln Co. High School
- Shelbyville Central High School
- Stewarts Creek High School
- Summit High School
Class 5A – Region 6
- Brentwood High School
Centennial High School - Clarksville High School
- Dickson Co. High School
- Henry Co. High School
- Northeast High School
- Rossview High School
Class 6A – Region 3
- Antioch High School
- Franklin High School
- John Overton High School
- McGavock High School
- Mt. Juliet High School
- Ravenwood High School
- Wilson Central High School
